Table 4.

Functional Data Obtained From Skeletal Muscle Mitochondria Isolated From Young and Mature Animals With Complex II–Linked Substrates, Succinate (10 mM) and Rotenone (4 μM)

	Young	Mature
State 3 (nmol O2/min/mit)	15.9 × 10−8 ± 9.0 × 10−8	3.6 × 10−8 ± 1.1 × 10−8*
State 4 (nmol O2/min/mit)	7.2 × 10−8 ± 4.1 × 10−8	2.3 × 10−8 ± 0.7 × 10−8*
RCR	2.2 ± 0.1	1.5 ± 0.1*
ADP/O per mit	1.15 × 10−9 ± 0.2 × 10−9	0.33 × 10−9 ± 0.03 × 10−9*

Notes: Data are mean ± standard deviation and represent 8 separate experiments in each group. State 3 and State 4 respiratory rates are expressed as nanomoles of O₂ consumed per minute per mitochondrion. RCR = respiratory control ratio; ADP/O = number of nanomoles of ADP phosphorylated by nanomoles of O₂ consumed per mitochondrion; mit = mitochondria.

^*Significantly different from young animals (p < .05).