Table 5.

Effect of Age on Olygomycin-Inhibited State 3 Respiration and CCCP-Induced Uncoupled Respiration in Skeletal Muscle Mitochondria Isolated From Young and Mature Animals

	Young	Mature
State Olygomycin (nmol O2/min/mit)	1.7 × 10−8 ± 0.9 × 10−8	0.5 × 10−8 ± 0.1 × 10−8
State CCCP (nmol O2/min/mit)	9.4 × 10−8 ± 5.2 × 10−8	2.14 × 10−8 ± 0.6 × 10−8*

Notes: Data are mean ± standard deviation and represent 8 separate experiments in each group. Results are expressed as nanomoles of O₂ consumed per minute per mitochondrion. Respiration was induced with pyruvate (5 mM) and malate (2 mM) as energizing substrates and saturated ADP concentration (1 mM) to initiate State 3 respiration. State 3 was inhibited after the addition of olygomycin (final concentration 1, 5 μg/mL) and CCCP (final concentration 2 μM) to uncouple mitochondrial respiration. CCCP = m-chlorophenylhydrazone; mit = mitochondria.

^*Significantly different from young animals (p < .05).