Figure 1.

Single-channel activity in the basolateral membrane domain of polarized epithelial cells isolated from NGB. (A) Cell-attached patch, V_p (pipette voltage) = 0. There is low-level K⁺ channel activity under control conditions, with a large increase after rapid application of −1.5 kPa to the pipette interior (syringe connected to water manometer). Note the rapid reversibility of the effect. (B) Changes in steady-state channel activity by −1.2 kPa. (B, Right) Current histograms are depicted; bin = 0.24 pA. (C) Effect of hyposmotic cell swelling (HYPO Δ osmolality = −28%) on K⁺ channel activity. (C, Right) Current histograms are depicted; bin = 0.20 pA. The bath solution was changed slowly; HYPO data shown were obtained near maximum swelling.