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. Author manuscript; available in PMC: 2009 Jun 4.
Published in final edited form as: J Struct Biol. 2007 Feb 3;159(1):149–153. doi: 10.1016/j.jsb.2007.01.015

Figure 1. 2D crystals of SecA ring-like structures.

Figure 1

A. The negative stained 2D crystals of SecA formed on E. coli lipid monolayers. The crystallization condition was 10 mM Tris-Ac, pH 7.4, 200 μg/ml SecA, incubating with lipid monolayer composed of E. coli lipid extract for 24 hour at 4°C. The scale bar is 100 nm.

B. Computed Fourier transform of a SecA 2D crystal after two-round unbending. The resolution circles representing 20, 15 Å were indicated. Only spots with IQ less than 4 are displayed, and the size of the spot is inverse proportional to IQ: IQ 1 is largest and IQ 4 is smallest.

C. The projection map of SecA 2D crystals averaged from 10 untilted images without any symmetry imposed. The scale bar is 5 nm.