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. Author manuscript; available in PMC: 2009 Jun 4.
Published in final edited form as: Immunity. 2007 Dec 27;28(1):122–133. doi: 10.1016/j.immuni.2007.11.017

Figure 3. FTY720-mediated inhibition of T cell egress is partially CCR7 dependent.

Figure 3

(A) Flow cytometric analysis of CD45.2+ Ccr7-/- and CD45.1+ WT T and B cells in mixed bone marrow chimeras treated for 24 h with saline or FTY720 and with integrin blocking antibodies for 20 h. Numbers indicate percent cells in the indicated gates out of total T or B cells. The means (±SD) of lymphocyte numbers in the samples were as follows: saline lymph=6.3×103/ul (± 4.5×103), n=8; FTY720 lymph=7.5×102/ul (± 9.7×102), n=9; saline LN=1.3×107 (± 4.0×106), n=8; FTY720 LN=1.6×107 (± 8.1×106), n=9. (B) Frequency of CD45.2+ T cells in the peripheral LN, mesenteric LN and lymph of mice that had been reconstituted as in A (Ccr7-/- mixed chimera) or with a mixture of CD45.2+ Ccr7+/+ and CD45.1+ WT bone marrow as a further control (Ccr7+/+ mixed chimera). (C) Same data as in B plotted as the ratio of each type of T cell in lymph versus peripheral lymph nodes for each animal. Bars in B and C represent mean and points individual animals and the data are pooled from three experiments.