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Effect of urea and GPC on Km of human aldose reductase for d-xylose (n = 3). ∗, Significantly different from control (P < 0.05). Additional significant differences with the C298A mutant are urea versus GPC and GPC versus urea + GPC. Mean control values of Km are wild-type, 7.8 mM, and C298A, 191 mM.
