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Effect of urea and betaine on Km of human aldose reductase for dl-glyceraldehyde (n = 3). ∗, Significantly different from control (P < 0.05). All other differences are also significant with wild type. With C298A, mutant urea versus betaine and urea versus urea + betaine are significantly different. Mean control values of Km are wild type, 0.15 mM, and C298A, 1.12 mM.
