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Effect of urea and GPC on Km of human aldose reductase for dl-glyceraldehyde (n = 3). ∗, Significantly different from control (P < 0.05). Additional significant differences with wild type are urea versus urea + GPC and GPC versus urea + GPC. Additional significant differences with C298A are urea versus GPC and urea versus urea + GPC. Mean control values of Km are wild type, 0.17 mM, and C298A, 0.94 mM.
