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. 2009 Jun 16;4(6):e5928. doi: 10.1371/journal.pone.0005928

Figure 6. Functional characterization of the CD4/10.4 and CD8/10.4 T cells at different stages of infection.

Figure 6

(Early: week 6; Intermediate: week 16, late: week 40) (A and B) Lung cells from infected mice were stimulated in vitro with TB10.4 3–11 or TB10.4 74–88 (lower panel) or left non stimulated (“Media”, upper panel) in the presence of αCD107a/b and stained with αIFN-γ and αCD8 (A), or αCD4 (B), antibodies (C) CD107a/b MFI of the IFN-γ positive cells following in vitro stimulation with TB10.4 3–11 or TB10.4 74–88. (D) The specific lysis of TB10.4 3–11 or TB10.4 74–88 loaded cells was determined in an in vivo cytotoxicity assay. Unloaded splenocytes (CFSElow) and TB10.4 3–11 or TB10.4 74–88 loaded splenocytes (CFSEhigh) from naïve mice were transferred into infected mice. The amount of splenocytes killed in vivo by cytotoxic T cells specific for either TB10.4 3–11 or TB10.4 74–88 was observed as a reduction in the CFSEhigh population and a percent specific lysis was calculated. A value of P<0.05 (students paired t-test) was considered significant and is shown by *.