Figure 1.

Yeast two-hybrid assay of interactions between NPR1 and bZIP transcription factors. (A) Yeast cells (EGY48) containing TomNPR1 and NIF1, NPR1 and AHBP-1b, NPR1 and TGA6, or NPR1 and OBF5 were grown for 2 days at 30°C on galactose medium lacking leucine for detection of the LEU2 reporter expression. (B) β-Galactosidase activity was calculated as described previously (33). Three independent colonies were taken for each combination, and four measurements were performed on a culture grown from each colony. The mean ± SD is presented.