Skip to main content
. 1999 May 25;96(11):6523–6528. doi: 10.1073/pnas.96.11.6523

Figure 3.

Figure 3

In vitro analysis of interactions between NPR1 and AHBP-1b or OBF5. Immunoblot analysis was performed on protein preparations purified by using Ni-NTA resin to detect copurification of NPR1 with His-tagged AHBP-1b or OBF5. The blot was probed with an antiserum generated against the carboxyl-terminal 16 aa of NPR1. Lanes: 1, crude extract (0.2 μl) of Sf9 insect cells expressing NPR1 by baculovirus; 2, NPR1 copurified with AHBP-1b; 3, NPR1 copurified with OBF5; 4, NPR1 copurified with AHBP-1b from a separate purification; 5, NPR1 purified with Ni-NTA resin alone.