Figure 8.

Time dependency of the inhibition of GAPDH by IAA or IA in cultured astrocytes. The cells were incubated for up to 60 min without (circles) or with IAA (A) or IA (B) in concentrations of 0.1 mM (triangles), 0.3 mM (squares) or 1 mM (diamonds). The results represent mean ± SD of data that were obtained on three independently prepared cultures that contained 45 ± 5 μg cytosolic protein per well. The initial specific GAPDH activity (100%) corresponded to 492 ± 45 nmol/(min × mg) cytosolic protein. The significance of differences to the data obtained for the control condition (absence of inhibitor) are indicated by **p < 0.01 or ***p < 0.001.