Table 2.

Specific activities of enzymes in cultured astrocytes after exposure to IAA or IA.

Enzyme	Inhibitor	Enzyme activity (nmol/(min × mg))	n
GAPDH	None	339 ± 145	6
	IAA	8 ± 13***	6
	IA	4 ± 4***	6
G6PDH	None	108 ± 12	3
	IAA	96 ± 8	3
	IA	104 ± 10	3
GR	None	56 ± 3	3
	IAA	25 ± 12*	3
	IA	40 ± 6	3
LDH	None	3278 ± 374	3
	IAA	3798 ± 379	3
	IA	3832 ± 331	3

Primary astrocyte cultures were incubated in the absence or the presence of 1 mM of IAA or IA. After 60 min incubation, the cells were lysed and the specific activities of the enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glucose-6-phosphate dehydrogenase (G6PDH), lactate dehydrogenase (LDH) and glutathione reductase (GR) were determined. The results represent mean ± SD of data that were obtained on n independently prepared cultures that contained 45 ± 5 μg (n = 3) or 37 ± 11 μg (n = 6) cytosolic protein per well. The significance of differences to the data obtained for the control condition (none) is indicated by *p < 0.05 or ***p < 0.001