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. 2009 Jun 15;4(6):e5922. doi: 10.1371/journal.pone.0005922

Figure 3. GPR3 expression during postnatal rat and mouse cerebellar development.

Figure 3

Panel a: Total mRNA was extracted from rat cerebelli (N = 12–15 per time point) at 4 different developmental stages (P1, P7, P21) and adult (7 to 8 weeks). RNA samples were subjected to quantitative RT-PCR analysis using probes and primers specific to rat GPR3. Data were adjusted using β-actin mRNA as control. Panel b: To determine the distribution of GPR3 in the developing postnatal cerebellum, a GPR3−/−; LacZ +/+ mouse was employed, where the E. coli LacZ gene was substituted into the GPR3 locus and was thus under transcriptional control of the endogenous GPR3 promoter. Staining for β-galactosidase expression revealed increased activation of transcriptional activity of the GPR3 promoter in the internal granular layer of cerebellum from P5 to P12 stages of postnatal development. Scale bar = 100 µm.