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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: FEBS Lett. 2009 Apr 11;583(9):1521–1527. doi: 10.1016/j.febslet.2009.04.007

Figure 1. Over-expression of pro-inflammatory factors and Up-regulation of ER stress markers in the retinas of Akita mice.

Figure 1

A). Western blot analysis of TNF-α and VEGF in the retinas of Akita mice. The membrane was reblotted with β-actin antibody as loading control. B). Retinal levels of TNF-α and VEGF were quantified by densitometry from 4 individual animals (mean ± SD, n = 4). C). mRNA level of GRP78 in the retina determined by real-time RT-PCR and normalized by 18S (mean ± SD, n = 7 in control group and n = 4 in Akita group). D). Representative images of GRP 78 expression in the retinas from 4 Akita mice and 5 littermate controls. Note more intensive signal of GRP 78 (brown color) in the inner retina of Akita mice (C-b) when compared to control (C-a). Magnification: 200x. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. E). Western blot analysis of phospho-IRE1α, phospho-eIF2α and ATF4 in the retinas of Akita mice. E). Retinal levels of phospho-IRE1α, phospho-eIF2α and ATF4 were quantified by densitometry (mean ± SD, n = 8). * p<0.05 and **p<0.01.