Figure 3.

Regeneration of myelinated and unmyelinated fibers. Neurofilament immunostaining labeled axons regenerating around the shaft of the electrodes [asterisks (A,B)]. Schwann cells (S-100+) were visualized along regenerated axons [β-tubulin+ (C,D)]. Both myelinated [(E) MBP+; red] and unmyelinated (pain) fibers [(F) CGRP+; green] were visualized in close proximity to the electrodes (dotted line). Nodes of Ranvier were observed near the electrodes [arrows in (E)]. Scale bars = 50 μm (A,C) and 25 μm (B,D–F).