Table 8.
Molecular Cytogenetic and Cytogenomic Techniques for Detection of Genomic Variations
| Techniques | Interindividual Genomic Variations | Intercellular Genomic Variations | Resolution |
|---|---|---|---|
| Banding techniques (karyotyping)1 | + | +/- | 3-5 Mb |
| FISH with site-specific/chromosome enumeration probes2,3 | + | + | >1 kb |
| SKY1 | + | +/- | >5 Mb |
| Multicolor banding (MCB)1or2 | + | +/- | 3-5 Mb |
| ICS-MCB3 | - | + | 3-5 Mb |
| BAC array CGH1 | + | - | ~1 Mb |
| Oligonucleotide array CGH1 | + | - | 30-50 kb |
| Single-cell array CGH1 | - | + | ~1 Mb |
| Single-cell sequencing1,4 | - | +/- | >1 bp |
| Single-cell SNP array1,4 | - | +/- | ~50 kb |
1
approaches studying the whole genome
2
approaches studying targeted DNA sequences or specific chromosomes
3
applicable for interphase cytogenetics and detects balanced chromosome abnormalities in interphase
4
poorly applicable for gross genomic variations.