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. 2009 Mar 26;23(6):925–931. doi: 10.1210/me.2008-0298

Figure 5.

Figure 5

Let-7 regulates adipogenesis by decreasing HMGA2 expression. A, HMGA2 protein levels in 3T3-L1 cells were assayed by Western blot analysis 48 h after transfection with a let-7a precursor or control oligonucleotide. GAPDH is shown as a loading control. B, Hmga2 mRNA levels were evaluated by RT-qPCR at the indicated time points after treatment of 3T3-L1 cells with DMI (shown are data from triplicate samples ± sd). C, HMGA2 protein levels were evaluated by Western blot analysis at the indicated time points after treatment of 3T3-L1 cells with DMI. D, mRNA expression of various adipogenic genes was assayed by RT-qPCR in differentiated 3T3-L1 adipocytes that were treated with siRNAs for Hmga2 or a control sequence (n = 3 ± sem; *, P < 0.05; **, P < 0.01). Hmga2 levels were monitored 24 h after siRNA transfection and aP2 and Pparγ levels were monitored at 6 d after siRNA transfection and incubation with DMI.