Figure 8.

Ser/Thr residues required for PKA-dependent ERRα transcriptional activity. Putative Ser and Thr phosphorylation sites throughout ERRα were mutated to Ala. Expression vectors containing either wt ERRα or one of these mutations were cotransfected with the hSP-A_-291:LUC reporter ± an expression vector for PKAcatβ (panel A) or SRC-2 (panel B). phRL-TK expression plasmid was cotransfected to correct for transfection efficiency. Cells were harvested after 48 h, and lysates were assayed for firefly luciferase and Renilla luciferase activities. Data are presented as the mean ± sem of triplicate samples from a representative experiment repeated three times with similar observations. Con, Control.