Skip to main content
. 1999 May 25;96(11):6535–6540. doi: 10.1073/pnas.96.11.6535

Figure 1.

Figure 1

Physical map of pYLTAC7. (A) The map shows the location of some sites for endonucleases that cleave the molecular once or twice. LB and RB, left and right borders, respectively; OD, overdrive sequence; Pnos, promoter of the nopaline synthase gene; HPT, coding region of the hygromycin phosphotransferase gene; nos 3′, polyadenylation signals of the nopaline synthase gene; KanR, kanamycin-resistance gene (NPT1). The complete sequence of the vector is available in the GenBank database (accession no. AB020028). (B) Sequence of the cloning-site region upstream of the sacB gene. The primer sets (R1, R2, R3, L1, L2, and L3) are designed for isolation of end fragments of the inserted DNA by thermal asymmetric interlaced PCR (TAIL-PCR) (22).