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Stability of two TAC clones in E. coli and A. tumefaciens. TAC plasmid DNA of two independent clones were isolated from E. coli (DH10B) and were used for transformation of A. tumefaciens C58C1(MP90). The plasmid DNA in the Agrobacterium host was transferred back to the E. coli host. Digestion of plasmid DNA in each step is shown in this figure. (Left) A TAC clone digested by HindIII. (Right) Another TAC clone digested by PstI. M, molecular markers.
