Figure 4.
Complementation of the sgr1 mutation with large TAC clones of wild type. (A) The SGR1 locus was covered contiguously by TAC clones (13E18, 3P22, 20D10, 5I12, and 25N15) carrying large (ca. 80-kb) genomic DNA fragments of A. thaliana Columbia ecotype that were isolated by using two DNA markers, CDC2B and KSAP3. (B) Segregation of the T2 family seedlings of the transformed line E for gravitropic responses. Seedlings were grown in darkness for 3 days after germination with the plate setting as the direction of gravity indicated by g-1. The plate then was turned by 90° as indicated by g-2, and the seedlings were grown in darkness for 24 hr. About 75% of seedlings showed distinct negative gravitropic curvature in hypocotyls as wild type, whereas the remaining (marked by arrows) did not show gravitropic curvature at all (sgr1 mutation phenotype).