Table 1.
Transformation efficiency of A. thaliana with TAC clones
| Exp. | Construct | Transformants/ treated plants | Seeds screened/ transformant |
|---|---|---|---|
| 1 | 1 | 115/46 (2.5) | 770 |
| 2 | 265/43 (6.2) | 404 | |
| 2 | 1 | 268/48 (5.6) | 328 |
| 2 | 325/36 (9.0) | 246 | |
| 3 | 1 | 99/48 (2.0) | 970 |
| 2 | 98/48 (2.0) | 876 | |
| Sum (1 + 2 + 3) | 1 | 482/142 (3.4) | 565 |
| 2 | 688/127 (5.4) | 397 |
A TAC clone having an insert of 80 kb (construct 1) and the TAC vector without an insert (construct 2) were used for transformation of A. thaliana ecotype Wassilewskija. Numbers of parentheses are transformation efficiency. The results of three independent experiments and their sum are shown.