TABLE 2.

Common motifs among hotspot sequences lacking CRE hotspot

10-base motif number and sequence		Counta	8-base motif number and sequence		Count	7-base motif number and sequence		Count	6-base motif number and sequence		Count
1	GGATGTAAGT	3	1	TGACATCA	6	1	ACGTAAT	14	1	ACGTAA	22
2	GGTCTGGACC	3	4	CCAATGAG	4	2	ATGTCAC	9	6	ACATGA	16
			5	AGAGCTCTb	4	4	CCCCGCA	8	9	AAAGAT	15
			6	TCGGCCGAb	4	7	CCAATCA	7	10	GTATGA	15
			7	AGACGCAG	4	8	CCCACCC	7	45	CTATTA	11
			8	GTCTAGACb	4	15	CGTCATA	6	57	CATCCC	10
			9	ATAATTGG	4	31	CCCCCAC	5	21c	GATGAC	18
			10	AACAGGCG	4
			11	ATTGGCGG	4
			12	AAGCATGA	4
			13	CGCAGTAA	4
			21	AATGGATA	3
			22	CCATTACG	3
			41c	AGGGATGA	4

Some of the most common 6- to 10-base motifs among 398 sequences lacking the CRE core sequence, TGACGT. Only two motifs longer than 8-bp that occurred more than twice were found, 10-1 and 10-2. Only sequences tested for hotspot activity (Figure 3 and Table S4) are shown. Motif numbers correspond to those shown in Table S3a.

^aNumber of times that a given motif is found among the 398 sequences lacking the CRE-core sequence, TGACGT.

^bA palindrome. Palindromes are counted twice, because they occur on both strands.

^cThese motifs are found only among our total pool of sequences and are shown in Table S2a.