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. Author manuscript; available in PMC: 2010 May 1.

Published in final edited form as: J Vasc Surg. 2009 May;49(5):1296–1303. doi: 10.1016/j.jvs.2008.12.026

(A) siRNA to ADAM-9, -10 and -12 resulted in a ~50% decrease in their respective A Disintegrin And Metalloproteinase Domains (ADAM) protein expression compared to DMEM control or scrambled siRNA. This was specific. (B) Incubation with siRNA ADAM-9 and -10 significantly reduced EGFR activation in response to ATF. Scrambled siRNA and siRNA against ADAM-12 had no effect. (C) Incubation with GM6001, TAPI-0 and TAPI-1 and siRNA to ADAM-9 and -10 markedly decreased HB-EGF release from smooth muscle cells into the media. (D) Incubation with siRNA to ADAM-9 and -10 markedly decreased smooth muscle cells migration compared to scrambled siRNA in the Boyden chamber in response to ATF. Values are the mean±SEM (n=6, * p<0.05, **p<0.01 compared to control).

(A) siRNA to ADAM-9, -10 and -12 resulted in a ~50% decrease in their respective A Disintegrin And Metalloproteinase Domains (ADAM) protein expression compared to DMEM control or scrambled siRNA. This was specific. (B) Incubation with siRNA ADAM-9 and -10 significantly reduced EGFR activation in response to ATF. Scrambled siRNA and siRNA against ADAM-12 had no effect. (C) Incubation with GM6001, TAPI-0 and TAPI-1 and siRNA to ADAM-9 and -10 markedly decreased HB-EGF release from smooth muscle cells into the media. (D) Incubation with siRNA to ADAM-9 and -10 markedly decreased smooth muscle cells migration compared to scrambled siRNA in the Boyden chamber in response to ATF. Values are the mean±SEM (n=6, * p<0.05, **p<0.01 compared to control).