Figure 4.

Evaluation of enzymes in the target of rapamycin pathway in liver of caloric restriction (CR) and methionine-restricted (Meth-R) mice. Each group of immunoblots shows a representative experiment involving four ad libitum and four CR mice (top set) or four Meth-C and four Meth-R mice (bottom set), stained (top to bottom) for phosphorylated Akt, total Akt, phosphorylated mammalian target of rapamycin (mTOR), total mTOR, phosphorylated 4EBP1, and total 4EBP1. The bar graphics at the right show means and SEs of the mean for eight mice of each type. Asterisks indicate significance at p < .05 (*) or p < .01 (**) by an unpaired t-test for the indicated comparison.