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. 1999 May 25;96(11):6541–6546. doi: 10.1073/pnas.96.11.6541

Figure 5.

Figure 5

Analysis of AtHO1 mRNA levels in wild-type A. thaliana and the hy1 mutants. (A) RNA gel-blot analysis of total RNA (15 μg) isolated from Col wild-type plants and the hy1 mutants, hy1–1, hy1–100, and hy1–2. (B) AtHO1 mRNA levels in 6-day-old light-grown (L) or dark-grown (D) seedlings, and dark-grown seedlings exposed to continuous white light for 1 day (D to L). (Upper) RNA gel blot probed with an AtHO1 coding-region fragment. (Lower) Ethidium bromide-stained gel before transfer of the RNA onto the membrane. RNA loading was normalized by reprobing the blot with a β-tubulin cDNA (TUB). The ratio of the signals obtained with the AtHO1 and TUB probes was expressed as a percentage of that obtained for wild-type (A) or light-grown (B) plants and is shown below each lane.