Figure 6.

Transgenic complementation of the hy1 mutant phenotype. (A) Ler plants homozygous for the hy1–1 mutation were transformed with an empty vector or one containing a genomic fragment encompassing the entire AtHO1 gene. T₁ seeds were germinated on media containing kanamycin and grown for 14 days under continuous red light. Independently transformed antibiotic-resistant plants representative of hy1–1 harboring the empty vector or the AtHO1 gene are shown from the left to right, respectively. (B) The average hypocotyl length of hy1–1 seedlings transformed with the empty vector or one containing AtHO1 and the wild-type Ler parent transformed with a vector containing an unrelated protein (smGFP) (35). Each bar represents the average hypocotyl length (±SD) of at least 10 seedlings.