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. 2009 Mar 24;37(10):3243–3253. doi: 10.1093/nar/gkp190

Figure 2.

Figure 2.

LeishIF4G-3 comigrates with LeishIF4E-1 and LeishIF4E-4 by sucrose gradients analysis. Extracts of L. major cells grown at 26°C, and after exposure to 35°C for 18 h were loaded on 10–50% sucrose gradients. (A) Pattern of polysome migration on sucrose gradients. Fractions were collected from the top of the gradient and the O.D260nm was monitored. (26°C, continuous line; 35°C, broken line). Proteins were recovered by TCA precipitation, and immunoblotted with antibodies raised against LeishIF4E-1, LeishIF4E-4, LeishIF4G-3 as well as with anti-rpS6, for detection of fractions that contain complexes containing 40S particles. Western analysis was done on fractions of cells that were grown at 26°C (B) and 35°C (C).