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LeishIF4G-3-FLAG pulls down LeishIF4E-4. Leishmania major cells were transfected with pX-LeishIF4G-3-FLAG and cell lines expressing the FLAG tagged LeishIF4G-3 were selected in the presence of 200 μg/ml G418. The cells were lyzed by sonication and the soluble fractions (S) were incubated with anti-FLAG beads. After incubation for 3 h, the beads were washed (W), the proteins were eluted (E), separated by SDS–PAGE and analyzed by western blot.
