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. 2009 Mar 24;37(10):3243–3253. doi: 10.1093/nar/gkp190

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Binding of a LeishIF4G-3 peptide to LeishIF4E-1. (A) ¹⁵N, ¹HTROSY-HSQC spectra of ¹⁵N-labeled LeishIF4E-1 (250 µM) in the presence of m⁷GTP (5 mM) for stabilization (LeishIF4E-1 free, in blue). (B) Overlays of ¹⁵N, ¹HTROSY-HSQC spectra of ¹⁵N-LeishIF4E-1 free with increasing amounts of non-labeled LeishIF4G-3 peptide (NYLEPPYPGFSLDEVVRR). The concentrations of the LeishIF4G-3 added were 0 µM (blue, ratio 1:0), 250 µM (red, ratio 1:1) and 1.25 mM (green, ratio 1:5). (C) Enlargement of a region displaying the chemical shift differences between the spectra that is boxed in (B) in dashed lines. Peaks movements are indicated by arrows.