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Mapping interactions within the NTD by yeast two-hybrid. Segments of RAG1 (solid lines) fused to GAL4 DNA-BD were tested with the three binding targets. Ka = KPNA2, GM = GMEB1, SF = SF3A2. (+) represents growth on histidine drop-out plates in the presence of 7.5 mM 3-AT. Weak growth (W) represents growth with 5 mM 3-AT. (–) represents no growth. ND is not determined.
