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. 2009 Mar 25;37(10):3301–3309. doi: 10.1093/nar/gkp192

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — One-hybrid assay adapted to show GMEB binding to RAG proteins at the RSS. (A) The original one-hybrid assay (ref. 22). The RAG protein complex, fused to VP16, activates luciferase when bound to the 12RSS. (B) Modified one-hybrid assay is portrayed in which VP-16 is only present fused to GMEB1. Activation of luciferase requires protein–protein interaction between GMEB1 and the RAG complex on the 12RSS. (C) Luciferase activity (machine units) obtained from the listed combinations of proteins transfected into COS7 cells.