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. 2009 Mar 30;37(10):3354–3366. doi: 10.1093/nar/gkp210

Figure 2.

Figure 2.

Expression of the esp1396I genes in vivo. (A) The horizontal lines on the left panel show overnight 37°C growth of E. coli cells harboring a plasmid containing the entire Esp1396I system of without such a plasmid on an LB agar plate. Cells were spotted with indicated dilutions of λ-vir phage lysate. Two panels on the right show the results of primer extension analysis carried out with RNA purified from cells harboring a plasmid containing the entire Esp1396I system with a primer specific for esp1396ICR transcription unit (middle panel) or with a esp1396IM-specific primer (right panel). Primer extension products are indicated by arrows. (B and C) Top panels shows the results of overnight 37°C growth of E. coli cells harboring esp1396ICR (B) or esp1396IM (C) promoters fused to promotorless galK in the absence (1) or presence (2) or a compatible plasmid expressing C.Esp1396I on McConkey agar plates. Bottom panels show the results of primer extension analysis of RNA purified from cells shown at the top with a galK specific primer.