Table 1.
Oligonucleotides (adapters and primers) used in this study.
| Method | Oligonucletotide | Sequence | |
|---|---|---|---|
| 5’ RLM-RACE | Fgf-10 adapter | 5’-GCUGAUGGCGAUGAAUGAACACUGCGUUUGCUGGCUUUGAUGAA | |
| Nested PCR for 5’ RLM-RACE | Forward outer primer | 5’-GCTGATGGCGATGAATGAACACTG | |
| Forward inner primer | 5’-CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG | ||
| Reverse inner primer | 5’-CTGGGAACGCCAGCAGAAAAGGGA | ||
| Reverse outer primer | 5’-GGTGGGACATCTGAAACCCT | ||
| Cloning of human PEA3 | Forward primera | 5’-ATGGATCCAGGATGGAGCGGAGGATGAAA | |
| Reverse primera | 5’-CCCTCTAGACTAGTAAGAGTAGCCACCCT | ||
| RT-PCR and real-time RT-PCR | Mouse & human HPRTb | fwd | 5’-CCTGCTGGATTACATTAAAGCACTG |
| rev | 5’-GTCAAGGGCATATCCAACAACAAAC | ||
| Human GAPDH | fwd | 5’-CCATGGAGAAGGCTGGGG | |
| rev | 5’-CAAAGTTGTCATGGATGACC | ||
| Mouse Fgf-10 | fwdc | 5’-CGCAATTAGCAGGAGCTGCAG | |
| rev | 5’-GGTGGGACATCTGAAACCCT | ||
| Human Fgf-10 | fwd | 5’-ATGTCCGCTGGAGAAAGCTA | |
| rev | 5’-CCTCTCCTTGGAGCTCCTTT | ||
| Human GAPDHd | fwd | 5’-GACAGTCGGAAACTGGGAAG | |
| rev | 5’-GGCTGCAGGAGAAGAAAATG | ||
| Mouse Pea3 | fwd | 5’-AAACAGGAGCGCACAGACTT | |
| rev | 5’-GCCTGTCCAAGCAATGAAAT | ||
| Human Pea3 | fwd | 5’-CTGAGATCCTCTGGCACCTC | |
| rev | 5’-CTGAGTCGTAGGCGAAGTCC | ||
| Mouse Fgf-10e | fwd | 5’-AATTCGGAAAGCACGCGGAC | |
| rev | 5’-GGTGGGACATCTGAAACCCT | ||
| Mouse Fgf-3f | fwd | 5’-CTGTCTCACAGGATCACTAC | |
| rev | 5’-CTTGAAGCTGTAAAGGCATGC | ||
a
Engineered BamHI and XbaI recognition sites, respectively, are given in bold.
b
excluding genomic DNA
c
where transcription starts
d
Primers in the promoter region
e
directly upstream of the predicted transcription start site
f
genomic DNA control primers