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. 2009 Jun 16;4(6):e5940. doi: 10.1371/journal.pone.0005940

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Mendum et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Total N. meningitidis proteins separated by 2D gel electrophoresis using (a) a non-linear pI 4–7 1^st dimension and (b) a non-linear pI 6–9 1^st dimension. Gels were silver stained and replica gels western blotted with patient sera. Spots that were recognised by one or more sera on western blots are circled. Spots whose identity was determined are numbered in black, those that remain unidentified in grey.