Table 3. Proteins that bound IgG from one or more of the patients sera.

NMB	Name	Protein	Confirmeda	Literature
0052	Pilin retraction protein	PilT-1	nd
0131	50S ribosomal protein L7/L12	RplL	Y	Evidence for a surface role in N. gonorrhoeae during cell invasion [23], [24]
				Immunoreactive in Chlamydia trachomatis [25]
				Protective against Brucella abortus in mouse model [26]
				Neisserial L7/12 shown to be immunogenic but with limited immunogenicity for T cells [27]
				Identified on the surface of Streptococcus oralis [28]
0138	Elongation factor G	FusA	Y	Thermus thermophilus elongation factor G shown to be putatively membrane associated [29]
				Proposed as a diagnostic antigen in Helicobacter pylori [30].
				Identified on the surface of Streptococcus oralis [28]
0139	Elongation factor Tu	TufA	Y	Fibronectin binding in Mycoplasma pneumoniae [31]
				Cell wall associated in Mycobacteria leprae [32]
				Surface associated and associated with cell adhesion in Lactobacillus johnsonii [33]
				Bovine IgG binds Ef-Tu on surface of Anaplasma marginale [34]
				Surface located virulence factor on Pseudomonas aeruginosa, binding factor H and plasminogen [35]
				Immunoreactive in Chlamydia trachomatis [25].
0143	50S ribosomal protein L4	RplD	N
0336	Enoyl reductase	FabI	Y
0382	OMP class 4	RmpM	Y	Well documented surface protein [36].
0427	FtsZ	FtsZ	Y	Immunogen in convalescent Borrelia burgdorferi patients [37]
				A structural homolog is antigenic in Bartonella bacilliformis [38]
0530	Beta-hexosaminidase	NagZ	nd
0546	Alcohol dehyrogenase	AdhP	nd	Immuno-reactive in patients with systemic candidiasis [39]
0554	DnaK protein (hsp)	DnaK	nd	Immunoreactive and partially protective in mouse model for Bruclla abortus [40]
				Localized to the surface in Neisseria meningitidis [41]
				Immunoreactive in Chlamydia trachomatis [25].
0574	Glycine cleavage system T protein	GcvT	Y
0631	Phosphate acetyltransferase	Pta	N
0671	Malate oxidoreductase	SfcA	Y	Found in the immunoproteome of Brucella abortus [42]
				Putative serodiagnostic with Mycobacterium tuberculosis [43]
0763	Cysteine synthase	CysK	Y	Found in the immunoproteome of Brucella abortus [42]
0768	Pilin retraction protein	PilT-2	Y
0823	Adenylate kinase	Adk	Y	Identified on the surface of Streptococcus oralis [28]
0876	Ribosomal protein L25	RplY	Y	Antigens found in patients with borreliosis [44]
0959	Succinyl coA synthase	SucC	Y
1201	IMP dehydrogenase	GuaB	Y	Immunoreactive and protective in mice infected with Candida albicans [22]
1301	30S ribosomal protein S1	RpsA	Y	Immunoreactive in Chlamydia trachomatis [25]
1344	OMP p64k, PDH, E3 component	LpdA2	Y	Outer membrane location in Neisseria [45]
				Recognised by sera from convalescent neisserial patients [46]
1429	OMP PorA	PorA	nd	Well documented OMP
1572	Aconitate hydratase 2	AcnB	Y	Immuno-reactive in patients with systemic candidiasis [39]
1581	Histidinol dehydrogenase	HisD	Y
1613	Fumarate hydrytase	FumB	nd	Found in the immunoproteome of Brucella abortus [42]
1934	ATP synthase F1 beta chain	AtpD	Y
1972	Hsp60	GroEL	Y	Conserved proteins that are immunogenic in many bacterial pathogens
2002	Elongation Factor Ts	Tsf	nd	Identified on the surface of Streptococcus oralis [28]
2015	Hypothetical	-	N
2155	e transfer flavoprotein, beta subunit	EtfB	nd
2156	Heptosyltransferase I	RfaC	nd
n/a	Opa900(FAM18), not in MC58	n/a	nd	Well documented OMP

^aRecombinant proteins that were confirmed as binding their identifying sera are indicated (Y), those that failed to bind their identifying sera are indicated (N) and those that were not determined, either because no recombinant protein was generated or because no more sera was available are indicated (nd).