Fig. 2.

Expansion of human B lymphocytes following adenoviral transduction. Normal human B lymphocytes were infected with recombinant adenovirus encoding enhanced yellow fluorescent protein (EYFP), c-src dominant-positive (DP) or c-src dominant-negative (DN) and cultured in the CD40/CD154 system for 19 days. Cell counts were evaluated in triplicate by Trypan blue dye exclusion every 2 days and total expansion was determined. Data shown are representative of three experiments, performed with B lymphocytes isolated from different donors. Expansion factor and rate are indicated.