Fig. 3.

Signal transducer and activator of transcription 5b (STAT5b) mRNA expression in transduced human B lymphocytes. Normal human B lymphocytes were infected with recombinant adenovirus encoding enhanced yellow fluorescent protein (EYFP), c-src dominant-positive (DP) or c-src dominant-negative (DN) and cultured in the CD40/CD154 system. Expression of STAT5b was analysed 48 h post-infection. mRNA levels were determined by quantitative competitive–polymerase chain reaction (Q–PCR). Fold expression of the transcripts were expressed relative to transcripts level in Ad5/F35-EYFP infected B lymphocytes. All data represent the means of three independent experiments; standard deviations are indicated.