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. 2009 Jun;156(3):471–478. doi: 10.1111/j.1365-2249.2009.03919.x

Fig. 1.

Fig. 1

Kinetics of immunoglobulin (Ig)E, IgA and IgM antibodies induced in rats after intraperitoneal inoculation with live (closed circles) or dead (open circles) L3 Anisakis larvae. Enzyme-linked immunosorbent assays were carried out with recombinant Ani s 7 allergen (t-Ani s 7), native Ani s 7 (nAni s 7) or crude extract (CE) as target antigens. Each serum sample was tested in duplicate. Cut-off values are represented by a horizontal dashed line.