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Kinetics of immunoglobulin (Ig)G (IgG1, IgG2a, IgG2b and IgG2c) antibodies induced in rats after intraperitoneal inoculation with live (closed circles) or dead (open circles) L3 Anisakis larvae. Enzyme-linked immunosorbent assays were obtained with recombinant Ani s 7 allergen (t-Ani s 7), native Ani s 7 (nAni s 7) or crude extract (CE) as target antigens. Each serum sample was tested in duplicate. Cut-off values are represented by a horizontal dashed line.
