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Kinetics of immunoglobulin (Ig)G1, and IgE antibodies induced in rats after intraperitoneal challenge (secondary response) with live (closed circles) or dead (open circles) L3 Anisakis larvae. All the enzyme-linked immunosorbent assays were carried out with recombinant Ani s 7 allergen (t-Ani s 7) as target antigen. Each serum sample was tested in duplicate. Cut-off values are represented by a horizontal dashed line.
