Fig. 3.

Mycobacterium leprae infection reverses peptidoglycan (PGN)-induced reduction of tryptophan aspartate-containing coat protein (TACO; also known as CORO1A or coronin-1) protein levels. Human promonocytic cell line (THP-1) cells (1 × 10⁶) were cultured in a six-well plate, treated with 20 ng/ml of phorbol myristate acetate (PMA) for 48 h, and stimulated with 2 µg/ml of PGN and/or M. leprae (multiplicity of infection: 10). After incubating for the indicated time, total RNA and total cellular protein were purified and reverse transcription–polymerase chain reaction analysis (a,c) and Western blot analysis (b,d) were performed. Densitometric analysis of the specific bands detected in the Western blot is shown in a bar graph.