Fig. 4.

5-amino-4-imidazole carboxamide riboside (AICAR) does not inhibit inhibitory kappa B (IκB) phosphorylation or nuclear factor (NF)-κB translocation in lipopolysaccharide (LPS)/interferon (IFN)-γ-stimulated mesangial cells. Mesangial cells from 8-week-old MRL/lpr mice were pretreated with or without AICAR (1 mM) for 24 h and stimulated with LPS/ IFN-γ for 15 min to 4 h. (a) Cellular protein was assessed for IκB protein and phosphorylated IκB protein expression by Western blot using anti-IκB-α and anti-IκB-α-p. (b) Nuclear protein was collected and assessed for p65 protein expression by Western blot. Blots are representative of three experiments.