Table 1.

5-amino-4-imidazole carboxamide riboside (AICAR) administration and cell viability.

	0 mM (control)	0·05 mM AICAR	0·1 mM AICAR	0·5 mM AICAR	1·0 mM AICAR
Necrotic	8·71 ± 2·38	9·17 ± 1·55	6·64 ± 2·43	6·92 ± 1·94	13·76 ± 9·46
Necrotic/apoptotic	19·60 ± 4·21	18·24 ± 3·71	20·16 ± 3·23	28·48 ± 4·35	37·37 ± 3·91
Live	63·96 ± 6·30	64·60 ± 4·82	64·76 ± 5·22	55·40 ± 5·30	39·48 ± 8·35
Apoptotic	7·89 ± 1·01	7·97 ± 1·52	8·46 ± 0·76	9·17 ± 1·82	9·37 ± 1·05

Mesangial cells were treated with AICAR at the concentrations indicated for 16 h and apoptosis and necrosis were determined using annexin V/propidium iodine staining and analysed by flow cytometry. Data are shown as percentage of total cells.