Figure 7. Survival kinetics of M. smegmatis (with M. tuberculosis gyrA-AS and gyrB –AS plasmids individually).
Induction at 10 uM IPTG concentration. In the case of gyrA-AS r-M. smegmatis the cfu reduction is >3 log10 (bactericidal target), but gyrB-AS showed no cfu reduction as the orphan gyrB takes over once the other gyrB transcript is blocked.