Fig. 3.

Mucilage release of men4-1 versus wild-type seeds in ruthenium red with and without shaking. (A, B) Col-2 wild-type seeds. (A) Seeds put directly into ruthenium red without shaking are surrounded by an outer, diffuse layer of mucilage and an inner, dense layer of mucilage, while those shaken in dye lose the soluble outer layer (B). (C, D) men4-1 seeds lack mucilage release when treated directly with ruthenium red, with or without agitation. Scale bar: 500 μm. (This figure is available in colour at JXB online.)