Fig. 5.

Seed coat phenotype of men2 mum4, men6 mum4, and control seeds following extraction with ammonium oxalate. (A) Seeds extracted with ammonium oxalate with shaking at 30 ^oC, then stained with ruthenium red. Note substantial mucilage release for Col-2 seeds. mum4-1 seeds release less mucilage, with the outer wall appearing to remain largely intact. (B) Scanning electron microscopy of air-dried ammonium oxalate extracted seeds. Only Col-2 seeds show obvious rupture of outer primary cell wall. Scale bars: (A) 10 μm, (B) 25 μm. (This figure is available in colour at JXB online.)