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Complementation of Saccharomyces cerevisiae strain LR2 deficient in the uptake of di- and tripeptides. Growth of LR2 cells expressing the peptide transporters AtPTR2 (positive control), HaPTR4, HaPTR5, and HaPTR12, and the strain transformed with the vector pDR196 (negative control) is shown. (A) MM medium containing 3.3 mM Arg-Glu, (B) SC medium containing 1 mM His-Ala, (C) SC medium containing 20 mM histidine. (D) Schematic representation of the strains used in (A–C).
