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. 2009 Apr 20;60(9):2665–2676. doi: 10.1093/jxb/erp111

Table 1.

Dry weight and number of cluster roots of hydroponically grown Hakea actites

N source Concentration (μM) Dry weight
No. of cluster roots per pot
Shoot (g) Roots (g) Cluster roots (mg) Cluster root (% of root dry weight) Total biomass (g) 13 weeks 22 weeks 23 weeks 24 weeks 25 weeks 26 weeks
No N 0 1.8±0.14a 0.75±0.08a 100±66b 11.4±6.2 2.7±0.2a 1.3±0.9 3.0±0.6 3.3±0.9 7.7±0.3 13.0±2.6 17.7±2.7
NH4NO3 15 6.2±0.27b,c 2.1±0.4b 37a 1.9 8.4±0.3a,b 0 0.3* 0.3 1.3 1.3 1.3
150 47.0±4.0d 14.9±1.3d 0 0 62.1±5.2d 0 0 0 0 0 0
Glycine 15 9.3±1.5c 3.2±0.39b 35±28a 1.1±1.0 12.7±1.8b 0 0.3±0.3* 0.3±0.3 0.3±0.3 1.3±0.9 3.3±2.8
150 60.9±6.5e 15.1±1.7d 0 0 76.1±8.2e 0 0 0 0 0 0
BSA 15 3.9±0.2a,b 1.7±0.2a,b 49±27a,b 2.8±1.7 5.8±0.4a,b 0 1±1* 1±1 8.0±1.5 13.7±2.3 23.3±2.3
150 33.9±3.0f 9.6±0.2c 0 0 43.5±3.2c 0 0 0 0 0 0

Plants were grown without N for 3 months and grown for an additional 7 months with different N treatments. Low-N-treated plants commenced producing cluster roots 13 weeks after commencement of hydroponic culture with different N treatments, while plants grown in adequate N did not produce cluster roots. Phosphorus was omitted from the growth media after week 22. The average of three pooled seedlings in three pots ±SD is shown. Means marked with different letters are significantly different (P <0.05, ANOVA LSD post hoc test). The dry weight of plants was determined in week 26.

*

Cluster roots were produced by plants in one pot only.