Table 1.
N source | Concentration (μM) | Dry weight |
No. of cluster roots per pot |
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Shoot (g) | Roots (g) | Cluster roots (mg) | Cluster root (% of root dry weight) | Total biomass (g) | 13 weeks | 22 weeks | 23 weeks | 24 weeks | 25 weeks | 26 weeks | ||
No N | 0 | 1.8±0.14a | 0.75±0.08a | 100±66b | 11.4±6.2 | 2.7±0.2a | 1.3±0.9 | 3.0±0.6 | 3.3±0.9 | 7.7±0.3 | 13.0±2.6 | 17.7±2.7 |
NH4NO3 | 15 | 6.2±0.27b,c | 2.1±0.4b | 37a | 1.9 | 8.4±0.3a,b | 0 | 0.3* | 0.3 | 1.3 | 1.3 | 1.3 |
150 | 47.0±4.0d | 14.9±1.3d | 0 | 0 | 62.1±5.2d | 0 | 0 | 0 | 0 | 0 | 0 | |
Glycine | 15 | 9.3±1.5c | 3.2±0.39b | 35±28a | 1.1±1.0 | 12.7±1.8b | 0 | 0.3±0.3* | 0.3±0.3 | 0.3±0.3 | 1.3±0.9 | 3.3±2.8 |
150 | 60.9±6.5e | 15.1±1.7d | 0 | 0 | 76.1±8.2e | 0 | 0 | 0 | 0 | 0 | 0 | |
BSA | 15 | 3.9±0.2a,b | 1.7±0.2a,b | 49±27a,b | 2.8±1.7 | 5.8±0.4a,b | 0 | 1±1* | 1±1 | 8.0±1.5 | 13.7±2.3 | 23.3±2.3 |
150 | 33.9±3.0f | 9.6±0.2c | 0 | 0 | 43.5±3.2c | 0 | 0 | 0 | 0 | 0 | 0 |
Plants were grown without N for 3 months and grown for an additional 7 months with different N treatments. Low-N-treated plants commenced producing cluster roots 13 weeks after commencement of hydroponic culture with different N treatments, while plants grown in adequate N did not produce cluster roots. Phosphorus was omitted from the growth media after week 22. The average of three pooled seedlings in three pots ±SD is shown. Means marked with different letters are significantly different (P <0.05, ANOVA LSD post hoc test). The dry weight of plants was determined in week 26.
Cluster roots were produced by plants in one pot only.